Zero may refer to:
- Zero, a character from the video game, Drakengard 3.
- Zero, a character from the Kirby media franchise.
- Zero-Two, the final boss of the video game, Kirby 64: The Crystal Shards.
- Zero, a character from the video game series, Mega Man X.
- Zer0, a vault hunter from Borderlands 2.
- SMG0, the main villain of the SMG4 Genesis and Revelations arcs.