Zero may refer to:
- Zer0, a vault hunter from the video game, Borderlands 2.
- Zero, a character from the video game, Drakengard 3.
- Zero, a character from the Kirby media franchise.
- Zero-Two, the final boss of the video game, Kirby 64: The Crystal Shards.
- Zero, a character from the video game series, Mega Man X.
- SMG0 (Zero), the main villain of the Genesis and Revelations arcs of the television series, SMG4.
- Zero, The Main Villain Of The Red Vs Blue's 18th Season, 'Red vs Blue: Zero'.