Talk:SA-X/@comment-24750734-20150427181633/@comment-28856679-20150620185249

I'd have to say "No." for the following reasons:

The name of the One Minute Melee is "Samus Aran vs Mega Man", and there has also been multiple cameo characters in certain One Minute Melees:

Captain Falcon vs Captain Commando

Batman vs Albert Wesker

Bowser vs Dr. Eggman (due to the Koopa shell)

Deadpool vs Deathstroke (due to the man talking to Deathstroke before the fight)

Dante vs Ragna the Bloodedge (due to the dead bodies before the fight)

Sakuya Izayoi vs Dio Brando

Dan vs Hercule

Lucario vs Renamon (due to Sir Aaron's gravestone)

Then there's this episode along with Sonic the Hedgehog vs The Flash.